mouse monoclonal anti probdnf antibody  (Santa Cruz Biotechnology)

Journal: Biomedicines

Article Title: A Truncated Receptor TrkB Isoform (TrkB.T1) in Mechanisms of Genetically Determined Depressive-like Behavior of Mice

doi: 10.3390/biomedicines11092573

Figure Lengend Snippet: Characteristics of antibodies used.

Article Snippet: Mouse monoclonal anti-proBDNF antibody , 1:2500 , Overnight, 4 °C , 5% NFDM-TBST , Santa Cruz, Santa Cruz, CA, USA (Sc 65513).

Techniques: Incubation

Journal: The Journal of Biological Chemistry

Article Title: N -Glycosylation is required for secretion of the precursor to brain-derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structures

doi: 10.1074/jbc.RA119.009989

Figure Lengend Snippet: ProBDNF stably expressed in HEK293F cells is glycosylated. N-Glycosylation of proBDNF and the prodomain is documented by mass shift following deglycosylation with PNGase F: A, detection of proBDNF and BDNF by an antibody recognizing the BDNF region; B, detection of proBDNF and the prodomain by an antibody recognizing the prodomain region. The gel images were spliced as indicated by space to exclude samples not related to the study.

Article Snippet: Mouse monoclonal anti-human proBDNF antibody (1:10,000, GeneCopoeia, Rockville, MD, number H10001G-MA) recognizing the prodomain region, was used to detect the proBDNF and prodomain ( 10 ).

Techniques: Stable Transfection

Journal: The Journal of Biological Chemistry

Article Title: N -Glycosylation is required for secretion of the precursor to brain-derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structures

doi: 10.1074/jbc.RA119.009989

Figure Lengend Snippet: Relative abundance of the glycoforms of HEK293F-produced proBDNF and prodomain expressed as % of all identified glycoforms

Article Snippet: Mouse monoclonal anti-human proBDNF antibody (1:10,000, GeneCopoeia, Rockville, MD, number H10001G-MA) recognizing the prodomain region, was used to detect the proBDNF and prodomain ( 10 ).

Techniques: Produced

Journal: The Journal of Biological Chemistry

Article Title: N -Glycosylation is required for secretion of the precursor to brain-derived neurotrophic factor (proBDNF) carrying sulfated LacdiNAc structures

doi: 10.1074/jbc.RA119.009989

Figure Lengend Snippet: Impact of modulators of glycosylation on the BDNF/proBDNF ratio in HEK293F cells. Western blotting using antibodies to prodomain and mature BDNF regions was analyzed by densitometry for the following: A, intracellular BDNF to proBDNF ratio; B, secreted BDNF to proBDNF ratio; C, intracellular prodomain to proBDNF ratio; and D, secreted prodomain to proBDNF ratio. The representative Western blotting is labeled as the graphs: CTRL, control; CHLOR, 50 mm sodium chlorate; KIF, 1 μg/ml kifunensine; TNMC, 5 μg/ml of tunicamycin. Results are expressed as scatter plot (mean ± S.D., n = 3). *, p < 0.05; **, p < 0.01; ***, p < 0.001 compared with CTRL, one-way analysis of variance with Bonferroni adjustment.

Article Snippet: Mouse monoclonal anti-human proBDNF antibody (1:10,000, GeneCopoeia, Rockville, MD, number H10001G-MA) recognizing the prodomain region, was used to detect the proBDNF and prodomain ( 10 ).

Techniques: Western Blot, Labeling